Evolutionary diversification of bean beetles: climate-dependent traits and development associated with pest status

Midori Tuda

Kyushu University, Japan

A large proportion of beetle species in the genus Callosobruchus are economically important pests of stored, dry post-harvest beans of the tribe Phaseoleae. However, the evolution of this key feeding habit is poorly understood. Here, using the phylogeny of the Asian and African Callosobruchus based on three mitochondrial genes, we first test the extent to which the ability to use dry beans is affected by ancestral traits, or phylogenetic signals, and then test for associations with the following variables: (1) host taxa in the wild, (2) a suite of climatic variables (i.e. annual mean temperature and number of arid months) and (3) the presence/absence of endosymbiotic bacteria, Wolbachia. Our phylogenetic analysis shows that both dry-bean use and host-plant use exhibited high phylogenetic signals, and transitions in the two were significantly correlated. The ability to use dry beans was suggested to promote habitat shifts from tropical to temperate regions. Adaptation to arid climate was also associated with the ability to reproduce on dry beans. Thus, our analysis suggests that physiological adaptations to an arid climate and to Phaseolinae hosts both render beetles predisposed to become pests of cultivated beans. Adaptation to dry food can indirectly enhance cold hardiness. Adaptation to low ambient humidity may have further served as pre-adaptation to the use of seeds with low water content. Long arid seasons and the associated availability of legume seeds may tend to favor repeated reproduction. This study predicts that new stored-product pests will likely evolve under arid climate.

 

 

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